Brief test method for assembling a chamber clean room

　Abstract A by TS type cleanroom air settling bacteria testing, testing methods to assess the cleanliness of the clean room - sedimentation method.
     Keywords clean room cleanliness dust concentration test bacteria colony settlement
TS-type device is a clean room with air unidirectional flow (horizontal parallel flow) in laminar flow filtered and multi-stage purification, and then by air to the air supply unit will work area after purification, so repeated high-cycle work the cleanliness of the clean room. TS TSFL clean room is also equipped with a flat-type air shower, air shower is installed at the entrance of the clean room, the operator must enter the cleanroom air shower air shower pass. When air shower air pollution through middle and high efficiency air filters, filtered clean air through vents to blow high-speed rotation in the tangential direction of the human body, will be attached to blow off the dust on the overalls.
     The clean room decontamination area of ​​13m3; cleanliness level is 100 (≥0.5μ total grain dust ≤3.5 / L; the total air volume ≈6000m3 / h, fresh air, 5%; the average wind speed ≥0.35 workspace section m / s; ACH about 180 times / h; interior pressure ≥0.5 mm water column; noise <70 db (A House); Power of FL-1 type Airshower 10.1kW, 380V, DY- Type 1 air purification unit 1kW, 380V, 380V as well as 220V lighting and power socket. clean room wind leaching room on the electrical circuit by the control box, electrical box and is equipped with 450V 75A ammeter and voltmeter each piece. As the devices with clean and high, sealed, rigid, light and beautiful, easy to dust, but also in a relatively short period of time to build a high-level of clean working environment and other characteristics, it is widely used in electronics, precision instruments, meters, medical and requires a high degree of air cleanliness various production sites,; also commonly used in quality control, health and epidemic prevention, hospitals and other departments requires a high degree of clean air sterile rooms, operating room.
   Due to the large supply of power to the device, and therefore, should be regularly in use current, voltage meter to send the measurement test of the department, and note that the device is working Never overload of work, you should always observe the state when the device is working on the electrical control box Current current, voltage table indicates, the voltage and the actual use of the current, voltage is consistent, if the current use of the current indicated by the ammeter large and very small, it should immediately stop using the ammeter test measurement department sent and maintenance. Also note that the cleaning cleanroom wind shower room, before the experiment, after using 5% Lysol wipe filtered dilution air supply, return air purification unit, equipment wall, countertops and Airshower filter walls and vents, purification unit to remove the filter should be cleaned frequently to ensure air purification unit is working properly. Also, often for clean indoor air cleanliness test, the test needs some instruments, such as the level of cleanliness (clean indoor air dust concentration) required the determination of dust particle counter; sectional average wind speed required heat
    Ball anemometer to measure; the interior pressure manometer measurement required tilt decline; required precision sound level meter measurement noise and so on. Press the test methods measure the clean room, test results are accurate, but since the test method equipment required more, and the use of units of the device is generally impossible to have this test conditions, it is impossible according to the above method cleanroom testing. So, using the staff how to do the clean room air cleanliness aware of? How a man of pollution clean room? How to make the test results with real accuracy of it? Here's a pair of clean indoor air by settling bacteria testing to test results the easy way to determine the cleanliness of the clean room. The method - after sedimentation method is to collect microbial particles in the air through natural sedimentation principle on glass plates by high pressure to the bacteria, add 45 ℃ temperature, nutrient agar, mix Heart cooling, after some time under suitable conditions let breeding to visible colonies were counted to dish colonies to determine the number of viable clean environment microorganisms, and in order to assess the concentration of the dust clean room.
   First, the necessary equipment:
   High pressure to bacteria disinfection (disinfection when used in strict accordance with manual operation), incubator (incubator thermometer measurement department must send periodic verification), dishes (usually with Φ90mm × 15mm borosilicate glass dish), Medium - nutrient agar.
1. Medium formula
    Fold egg peptone 10g, beef extract 3g, sodium chloride 5g, agar 18g, distilled water 1000ml.
2. Production method
    The components other than agar deep in the distilled water, 15% sodium hydroxide solution depth approximately 2ml, correcting the pH to 7.2 to 7.4, after adding agar heated to boiling, agar dissolves, packed in Erlenmeyer flask, 121 ℃ autoclave 15min, cooled placed in a refrigerator and stored at 2 ~ 5 ℃.
   Second, the test procedure

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